The MF497-16 is a 25 mm diameter Bandpass Filter, specifically designed for YFP (Yellow Fluorescent Protein) Excitation. This filter, manufactured by Thorlabs, is a crucial component in various optical systems, particularly in fluorescence microscopy and other bioimaging applications.
The filter's primary role is to allow light within a specific wavelength range to pass through while blocking out unwanted wavelengths. The MF497-16 is designed to pass light with a Center Wavelength (CWL) of 497nm and a Bandwidth (BW) of 16nm. This precise wavelength selection is crucial for exciting YFP, ensuring optimal fluorescence emission.
The MF497-16 is made from UVFS, a high-quality optical material, and has a circular shape. It has a thickness of 5mm, which contributes to its durability and stability in optical systems. The filter's front and back are coated with "tho mf497-16", a proprietary coating developed by Thorlabs to enhance its performance and longevity.
The filter's geometry, including its 25mm diameter, is designed to fit standard optical systems. The cut-on wavelength is 489nm, and the cut-off wavelength is 505nm, further emphasizing the filter's specificity for the 497nm wavelength.