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MF497-16 25 mm Dia. YFP Excitation Filter; CWL=497nm; BW=16nm

MF497-16 25 mm Dia. YFP Excitation Filter; CWL=497nm; BW=16nm

Type
Filter
Subtype
Bandpass Filter
Brand
Thorlabs
Product webpage
Thorlabs products in the 3DOptix catalog

Properties

Shape
Circular
Diameter
25 mm
Cut Off Frequency
505 nm
Cut On Frequency
489 nm

Material

Name
UVFS
Dispersion Equation
Sellmeier
n (λ)
1.462494021793911
λ
497 nm
B1
0.6961663
C1
0.004679148 μm2
B2
0.4079426
C2
0.013512063 μm2
B3
0.8974794
C3
97.93400254 μm2

MF497-16 25 mm Dia. YFP Excitation Filter; CWL=497nm; BW=16nm

The MF497-16 is a 25 mm diameter Bandpass Filter, specifically designed for YFP (Yellow Fluorescent Protein) Excitation. This filter, manufactured by Thorlabs, is a crucial component in various optical systems, particularly in fluorescence microscopy and other bioimaging applications.

The filter's primary role is to allow light within a specific wavelength range to pass through while blocking out unwanted wavelengths. The MF497-16 is designed to pass light with a Center Wavelength (CWL) of 497nm and a Bandwidth (BW) of 16nm. This precise wavelength selection is crucial for exciting YFP, ensuring optimal fluorescence emission.

The MF497-16 is made from UVFS, a high-quality optical material, and has a circular shape. It has a thickness of 5mm, which contributes to its durability and stability in optical systems. The filter's front and back are coated with "tho mf497-16", a proprietary coating developed by Thorlabs to enhance its performance and longevity.

The filter's geometry, including its 25mm diameter, is designed to fit standard optical systems. The cut-on wavelength is 489nm, and the cut-off wavelength is 505nm, further emphasizing the filter's specificity for the 497nm wavelength.

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